Porteur
Claudia Oliveira
Session
2019
Co-porteur(s)
Marta Tacão
Titre
FLOW: Anthropogenic associations with agents of microbial genes dispersal in Estarreja´s environments.
Résumé
In the last 8 years the OHMi-Estarreja has played a crucial role by financing projects to study contaminants impacts and to minimize pollution effects, to protect and aware population from these hazards. Several projects were about hazards related with microbial communities residing in soil, water and vegetables, for example. Issues like bacterial metal(oid) tolerance and antibiotic resistance have been addressed.
Bacterial plasmids, are genetic agents of gene dispersal among bacterial communities and are acknowledged as main agents of bacterial adaptation. Also, plasmids dispersion and gene acquisition is triggered by environmental conditions such as anthropogenic inputs.
Thus, this project has two main aims: To carry out a systematic review about OHMi-Estarreja project´s outputs to identify acute contaminated sites that have been associated with features of the bacterial communities, particularly about metal(oid)s tolerance and antibiotic resistance. The second will be to evaluate the presence of plasmid genetic structures and of genes that can be of metal and antibiotic resistance, throughout the different contaminated environmental compartments.
This project will evaluate if there are plasmid specific groups that are widespread among anthopogenically impacted environments in Estarreja region, or if they are otherwise confined to specific compartments. Also, plasmid load and diversity will be evaluated throughout the different impacted sites identified. Ultimately we will obtain an Estarreja region map of plasmids distribution and associated genes among anthopogenically impacted environments that will allow to infer hotspots of plasmids and associated resistance genes, and hypothesize about routes of dispersion.
Type
Projet OHMs
OHM(s) concerné(s)
  • Estarreja
Disciplines
Biologie
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Porteur

Claudia
Oliveira
Cláudia Oliveira is an invited Professor from the Biology Department, University of Aveiro since 2013. She is a microbiologist with a background in biochemistry. Her research interests are about environmental bacteria and selective pressures involvement in antibiotic resistance and mobile genetic elements dissemination. Studied environments have included the Ria de Aveiro lagoon water system and wastewater systems.

Participants

Marta
Tacão
• Marta Tacão is a Junior Researcher at the University of Aveiro (Biology Department and CESAM) since 2019. Her research interests, and confirmed experience, are about antibiotic resistance and public health, antibiotic resistance mechanisms and dissemination, bacterial genomics and environmental resistome. The research profile fits well in the FLOW projects aims. She has experience in culture-dependent and independent methodologies for the analysis of complex bacterial communities and strains; Skills in genomics for handling large sequence data and analysis; genome assembly and annotation; whole-genome based analysis for taxonomic inference and resistome and mobilome description; experience in characterization of antibiotic resistant bacteria and resistance mechanisms, dissemination potential, virulence traits, metal tolerance and pathogenicity traits; quantification of genetic determinants of resistance; Experience on handling environmental samples from pristine waters, metal-contaminated soils or wastewaters, for microbiological studies. Also has experience in participating in FCT and OHMi funded projects. For more details: www.cesam.ua.pt/martat