Tânia Melo
Rosário Domingues, Pedro Domingues, Sara Anjos, Simone Colombo, Frederico Cerveira
Deciphering the plasma lipid profile to unveil potential markers for obesity: Reshaping the multifactorial Obesity-Lipid Relationship (LipOBioma)
The Aveiro and Estarreja municipalities have been highly active in the prevention of overweight and obesity, coordinating some initiatives with partnership of Associação Portuguesa Contra a Obesidade Infantil, Sociedade Portuguesa para o Estudo da Obesidade, and the support of local community. Alterations in lipid profile are intrinsically related with obesity, and obesity-related lipidome depends on different key factors. To our best knowledge and based on literature survey, the lipid profile signature of overweight/obese population of Portugal, and the outcome of different factors on that signature is still unrevealed. This proposal aims to be integrate in two topics proposed by OHM-Estarreja (2018): i) Public health and ii) Evolution of the relations between society and environment. Through an interdisciplinary approach and the involvement of Centro Hospitalar Baixo Vouga, E.P.E. Aveiro, this project team, involving Doctors, Biochemists and Pharmaceutical sciences, will screen plasma to characterize the lipidome profile variations of overweight and obese individuals from Estarreja and Aveiro targeting putative biomarkers for overweight progression; obesity prediction, prevention, and monitoring; contribute to the understanding how age, gender and environmental background shifts the lipidome signature; and to identify sensitive patterns of obesity-related risk for development of liver and cardiovascular diseases, and diabetes. We believe that this innovative lipidomics-based approach and the science based knowledge to be generated will be broadly disseminated and play a key role in the regional, national and international understanding of the molecular basis of obesity, its correlation with the development of other pathologies, and contributing to its prevention, diagnostic and monitoring.
Projet OHMs
OHM(s) concerné(s)
  • Estarreja


Tânia Melo (http://orcid.org/0000-0002-0508-4034) is graduated in Biochemistry by the University of Trás-os-Montes e Alto Douro (UTAD). In 2011, she concluded her Master in Biochemistry with minor in Biomolecular Methods from University of Aveiro (UA). In 2017, she received her Ph.D. degree in Biochemistry focused on nitroxidative lipidomics, namely in nitroxidized phospholipid and their biological effects, from UA. During her Ph.D. she worked as a trainee at Leipzig University (Germany) and CIB-CSIC (Spain), which provided her a consolidated expertise in mass spectrometry and molecular biology applied to health and disease. Currently, she is a postdoctoral researcher at the Mass Spectrometry Centre (Department of Chemistry & QOPNA) at UA. Her research interests are characterization of lipid profile from different biological matrices, the study of oxidative/nitroxidative modifications in lipids using mass spectrometry-based approaches, and cross reactions between lipids and other biomolecules. Tânia Melo has published 28 papers in international peer reviewed journals, 32 poster communications, and 14 oral communications in several national and international scientific meetings, has been a member of scientific projects and organizing committee of several scientific meetings, and was referee of 2 papers from international journals.


Frederico Cerveira was graduated by the Faculty of Medicine of University of Porto (1976). He is a staff member of the Hospital of Aveiro since 1987, being the Head Manager of the Clinical Chemistry sector, and having in the Hospital Career the degree of Senior Graduate Assistant. As doctor in the Clinical Pathology Service, Frederico was responsible for the integral training of doctors in the specialization phase. He is also responsible for the training in Clinical Chemistry of all the other internal doctors of the Clinical Pathology Service, as well as of the trainees coming from other Hospital Services. Frederico has collaborated with the Chemistry Department of University of Aveiro in research projects, being supervisor of several thesis of the master degrees in Biochemistry. He has also given support to specific hospital internships of University students. He participated in several specialization and medical career graduation jury. Since 19/07/2007, he is a member of “Direcção do Colégio de Especialidade de Patologia Clínica da Ordem dos Médicos”. He is the president of “Direcção da Associação Portuguesa dos Médicos Patologistas – APOMEPA” since 2015, a position already held from 2000 to 2004. He has the “Competência de Gestão de Serviços de Saúde”, by the “Ordem dos Médicos” He was the Head of Department of Clinical Pathology of Hospital of Aveiro from 2003 to 2008, and on this date he requested the cessation of duties. Since 1989, he is an associate and performs management duties in a private laboratory for clinical analysis.
Pedro Domingues is presently assistant professor with aggregation at Aveiro mass spectrometry center at University of Aveiro, Portugal. He received his BA in Pharmaceutical Sciences at University of Coimbra in 1990 and his PhD in Chemistry- Mass Spectrometry at Aveiro University in 2000. He has been working in mass spectrometry applications since 1996. His research is concerned with applications of mass spectrometry for the study of oxidative stress. Main work has been developed in the detection and characterization of free radicals by mass spectrometry, the study of oxidative stress in lipids and phospholipids and amino acids. Currently his special interests are the study and characterization of oxidized lipids, peptides and proteins using mass spectrometric approaches. He is author or co-author of more than 150 publications (ISI author identifier: E-5202-2010). He is the Director of the Master in Biochemistry of the University of Aveiro since 2010.
Rosário Domingues is graduated in Pharmaceutical Sciences, University of Coimbra (1990), received her Ph.D. degree in Chemistry (1998), and Habilitation in Biochemistry (2014), from the University of Aveiro (UA). Since 2014, she helds the contract of Assistant Professor with Habilitation in the Mass Spectrometry Centre of the Department of Chemistry & QOPNA (UA). She has over 25 years of research experience in the field of mass spectrometry, as specialist in lipidomics, glycomics and changes in biomolecules associated with oxidative stress. She participated in several juries of academic examinations, scientific committees of congresses, and in the supervision of 12 Post-Docs, 20 Ph.D. and 60 M.Sc. students. She coordinated and participated in several research projects funded by national and European programs (18 in total), has authored one book and six book chapters, and has over 220 articles published in international journals with referee.
Sara Anjos is graduated in Biochemistry by the University of Aveiro (UA). During the degree she worked in lipidomics and mass spectrometry, which allowed her to acquire knowledge in the area. From this work has resulted one poster communication in a National Meeting. Currently, she is concluding her master’s degree in Biochemistry with minor in Clinical Biochemistry at the UA. She is working on the determination of lipid profile changes in pediatric obesity using mass spectrometry-based approaches.
Simone Colombo received his integrated master of science (MSc) in Pharmaceutical Chemistry and Technology at University of Milan in 2015, with a thesis aimed to develop mass spectrometry (MS) methods for the analysis of biopharmaceutical proteins. He has been working as a pharmacist during his studies. After the graduation, he worked in the pharmaceutical industry as quality assurance assistant. Exploiting this transversal education in the field of pharmaceutical and life sciences (pharmacy, industry, academia) he undertook his PhD fellowship in biochemistry at the mass spectrometry center of University of Aveiro as part of the project MASSTRPLAN (Mass Spectrometry Training Network for Protein Lipid Adduct Analysis) funded through Marie Skłodowska Curie Actions – Innovative Training Networks (MSCA-ITN), where he is presently enrolled. The main research interests of his PhD fellowship include the MS-based analytical characterization of modified aminophospholipids, along with the study of their implications in immune-mediated inflammatory diseases (IMIDs).