Flavio Silva
Jan Jacob Keizer, Luísa Seuanes Serafim, Paulo Costa Lemos, Isabel Campos
DRuMBEAT - Dirt-based microorganisms towards soil bioremediation of multiple contaminants
While soils of Estarreja continue to be important sinks of persistent contaminants, existing remediation techniques are often ineffective and fail when addressing multiple contaminants. Biochar – biomass pyrolysed for enhancing carbon sequestration and other biomass productivity – presents a dual potential for improving soil decontamination: its exceptionally high porosity can both promote adsorption of contaminants and provide habitat conditions for microbial biofilm development. It is hypothesized that the microbiomes existing in contaminated soils present enhanced abilities to thrive under such adverse environments, thus allowing for a natural improvement of the ecosystem services provided by the soil. The proposed research aims at a novel technique for remediating soil contamination, through development of biochar-attached biofilms made of mixed microbial cultures taken from those “dirty” soils identified in Estarreja and stimulated to oxidize both organic and inorganic contaminants. The work programme encompasses: microbial fingerprinting of the contaminated soils, ex situ development of biofilms cultivated from those microbiomes onto biochar carriers and assessment of the contaminant uptake kinetics.
Projet OHMs
OHM(s) concerné(s)
  • Estarreja
Biochimie, Biologie, Chimie


Flávio Silva, FS (http://orcid.org/0000-0001-7025-0601) got his PhD in Environmental Sciences and Engineering in 2013 at the University of Aveiro. He is an environmental scientist and engineer with seven years of research in bioprocess technology and biorefinery concepts (energetic and material valorisation of waste streams). After PhD, he broadened his research into further environmental subjects to develop skills under a wider vision of ecosystem interactions, including earth surface processes, carbon and nutrient dynamics, integrated ecosystem services and soil remediation. Currently FS is a researcher at CESAM – Centre for Environmental and Marine Studies at the University of Aveiro, working on carbon/nutrient dynamics, soil biotechnology and resource recycling at both scientific and industrial interface levels. He worked as executive manager and field team coordinator in 1 national project and 2 European projects. He has co-supervised 17 MSc students and participated as jury examiner of 7 MSc theses. He is/was team member of 7 research projects (either European or national) and collaborator of 13 research projects (either European or national). He owns a granted patent, has authored 19 peer-reviewed papers (h-index 7, SCOPUS) and more than 30 oral or panel presentations in conferences. FS career has not been only focused on fundamental science but he also has advanced skills on R&D projects management, students mentoring, design of funding applications, as well as a strong expertise in collaboration between scientists and stakeholders, notably aiming at technology transfer.


Jan Jacob
Jan Jacob Keizer (http://orcid.org/0000-0003-4833-0415) has a PhD in Natural Sciences, Specialisation Plant Ecology, from the University of Amsterdam (2000) and has a long-standing record of research into land degradation in Portugal, focussing on the impacts of wildfires, post-fire forestry operations and agricultural practices on vegetation, soils, and hydrological and soil erosion processes. He is currently Assistant Reseacher at the Centre for Environmental and Marine Studies (CESAM) of the University of Aveiro, and Principal Reseacher (FCT-IF) as of 01/01/2017. He is chair of the EcoHydrology Research Group of CESAM, and has been coordinator of the earth surface processes (ESP) team (http://www.cesam.pt/teamsite) at the Department of Environment and Planning since 2005. The ESP team currently comprises 22 members, 13 of whom have a PhD degree, as well as 8 MSc students, of whom 3 are international students from Wageningen University, The Netherlands. JJK has much experience in supervising post-doctoral (16) and PhD (12) grant holders as well as MSc students (39). JJK also has extensive experience in coordinating R&D projects as well as project research teams at the University of Aveiro. This includes PI-ship of 6 FCT-funded projects (ongoing: FIRE-C-BUDs, MASSC), and UA-team coordinatorship of, amongts others, 2 QREN projects (e.g. TRANSFIBRA) and 2 EU-FP7 projects (ongoing: CASCADE, RECARE). JJK has by now authored 95 articles in international peer-reviewed journals (SCOPUS database), which have been cited more than 1100 times, giving rise to h-index of 20.
Isabel Campos (http://www.cesam.ua.pt/icampos) got her PhD degree in Marine and Environmental Sciences in the University of Aveiro in May 2016. She is presently working as researcher fellow of the European project RECARE – Preventing and Remediating degradation of soils in Europe through Land Care (Grant Agreement 603498), at the Centre for Environmental and Marine Studies (CESAM) and at the Department of Environmental and Planning, University of Aveiro. In the last 5 years Isabel Campos was a PhD Student of the Doctoral Programme on Marine and Environmental Sciences of the University of Aveiro and University of Oporto. She also worked as a researcher fellow of the project FIRETOX- Toxic Effects of Wildfires on Aquatic Systems (PTDC/AAG-GLO/4176/2012), supported by the Portuguese Science and Technology Foundation (FCT), at the National Institute of the Sea and Atmosphere (IPMA). Additionally, she collaborated in the project FIRECNUTS- Wildfire effects on topsoil carbon and nutrients stocks, dynamics and exports (PTDC/AGR-CFL/104559/2008) funded by the FCT. Isabel Campos has experience in quality control and management of laboratories. Her research interest are focus in the assessment of environmental contamination (mainly of nutrients, carbon, metals and polycyclic aromatic hydrocarbons) in different environmental compartments (soils, water, plants and organisms) and their impacts in the terrestrial and aquatic ecosystems. Isabel Campos is author of 3 papers and co-author of 1 paper published in international peer review journals and over 15 communications in international scientific meetings.
Luísa Seuanes
Luísa S. Serafim (http://orcid.org/0000-0001-5802-1777) is currently working as Assistant Professor at University of Aveiro and researcher of CICECO/UA – Aveiro Institute of Materials. She got her PhD in Biological Engineering in 2004 at FCT/UNL and has been working on the use of mixed microbial cultures for the treatment and valorization of industrial sub-products and wastes with a special focus on production of biopolymers (bacterial cellulose and polyhydroxyalkanoates), biosurfactants and biofuels. The research interests include the characterization of microbial biopolymers, optimization and control of bioprocesses, and microbial ecology of mixed cultures consortia. Since 2002 she authored/co-authored 35 scientific papers in international peer-reviewed journals, six book chapters and several communications in International and National Conferences. Luísa S. Serafim is supervising or has supervised six PhD students and 36 Master students. As member of the research team, she participated in ten national and in one European research projects.
Paulo Costa
Paulo C. Lemos (http://orcid.org/0000-0001-6094-0107) got his PhD degree in Biological Engineering, specialization on Microbial Technology from New University of Lisbon in March 2001. Presently he is Principal Investigator at Associated Laboratory for Green Chemistry (LAQV-REQUIMTE) from the branch located in Faculdade de Ciências e Tecnologia of Universidade NOVA de Lisboa. Since 2009 he is group leader of the “Microbial Ecology and Technology Lab”. MET Lab research interested are related with biological processes involving mixed microbial cultures for: Waste/Wastewater Treatment and Reuse; Biological Nutrient Removal (organic/inorganic); Bioremediation of contaminated soils with pyrethroides; Biopolymers Production; Microbial Ecology; Metabolic Studies using in vivo NMR. He has participated as Team Member in 7 National projects (JNICT, POCTI, POCI, PTDC), 1 European project (FP6) and 8 Bilateral collaboration projects with Italy and Morocco (4 GRICES/CNR; 4 GRICES/CNRS). He has also been Principal Investigator of 2 National funded projects (POCI, PTDC). Paulo C. Lemos is author of 42 papers in international peer review journals, 1 World patent, 7 books chapters, and more than 140 communications in national and international scientific meetings (23 Nat.; 120 Int.). He has supervision experience at the level of Post-Doc, PhD, Master, undergraduate students and research scholars. He has been a peer reviewer for more than 33 International Scientific journal and Member of the Editorial Board of Applied Environmental Microbiology (since 2013). Paulo C. Lemos has participated has evaluator for funding research proposal by Academia and Industry for national and International funding agencies (IAPMEI; ADI; ANI; ESF; STW; Fondazione Cariplo).