Porteur
Nuno Ferreira
Session
2017
Co-porteur(s)
Miguel Oliveira, Laura Guimarães
Titre
ENCASE - EpigeNetic traits: a CAse Study from organisms living near the Estarreja Chemical Complex.
Résumé
The vicinity of Estarreja Chemical Complex (ECC) has a long history of soil, surface and groundwater contamination. Here, solid wastes from operating industry have been deposited in a landfill and sludge park without treatment, releasing contaminants to its surrounding. Along with the inorganic contamination, organic compounds and pharmaceuticals resulting from agricultural and cattle activities also contribute to the overall contamination. Organisms inhabiting vicinity grounds, and the river, have adapted to survive in this hostile environment and can provide essential clues to be used in similar scenarios. By evaluating the somatic epigenetic variations of soil and water organisms it is possible to determine which mechanisms are involved in tolerance and plasticity. The proposal integrates OHM-Estarreja (2017) topic “Evolution of Biodiversity: evaluation of ecosystems’ modifications in Ria de Aveiro”.

The ENCASE project will collect soil and water samples from the vicinity of the ECC and will use them in exposure assays with terrestrial (isopods and anelids) and aquatic organisms (daphnids and fish embryos). This will allow comparing methylation percentage and expression of DNA (cytosine-5-)-methyltransferases (DNMT) from exposed and non-exposed organisms. Over the last decades, several studies indicated a role for methylation and DNMTs in epigenetic events, but their involvement in toxic responses and adaptation of wild organisms to environmental contamination is still poorly understood. Since different taxa present different methylation patterns the results will allow to determine how the epigenetic modifications are acting and may serve as base for future identification of other epigenetic traits in local organisms.
Type
Projet OHMs
OHM(s) concerné(s)
  • Estarreja
Disciplines
Biologie, Chimie, Écologie
Image

Porteur

Participants

Oliveira
Miguel
Guimarães
Laura